Learn How to Set Up, Operate & Perform Canine Progesterone Testing with the VetChroma 3

Welcome to the K9Reproduction VetChroma 3 Training Center. This guide walks you through the essentials of operating your VetChroma 3 progesterone analyzer, including equipment setup, blood sample preparation, proper pipetting, test-kit preparation, running a test, and troubleshooting.

The VetChroma 3 features a 7-inch touchscreen, built-in incubation, integrated printing, and the ability to process up to three tests simultaneously.

Whether you're just getting started or need a quick refresher, follow the instructions below to become more familiar with your equipment.

1. Getting Started with Your VetChroma 3

Before performing your first progesterone test, prepare a clean, organized workspace with the necessary equipment.

Equipment & Supplies Needed:

• VetChroma 3 Progesterone Analyzer

• Compatible VetChroma Progesterone Test Kit

• Microcentrifuge for sample separation, when required

• Adjustable Micropipette & Disposable Tips

• Blood Collection Tubes

• Vortex Mixer, when required by the assay

• Sample Tubes & Laboratory Supplies

Place the VetChroma 3 on a stable, level surface and connect the supplied power accessories. Turn on the analyzer and allow the system to initialize.

Verify that the correct progesterone assay and test-kit lot information are configured before testing. Complete any required instrument checks or quality-control procedures.

2. Preparing Your Blood Sample

Proper sample preparation is essential for consistent progesterone testing.

Serum Testing: Collect blood in an appropriate serum tube and allow it to clot completely before centrifugation. Spin the sample using the correct settings for your centrifuge and collection tube.

After centrifugation, the clear yellowish liquid at the top is the serum. Carefully collect this portion without disturbing the packed blood cells below.

Plasma Testing: When the assay permits plasma, collect blood in a compatible anticoagulant tube and centrifuge to separate the plasma from the blood cells.

Whole Blood Testing: Compatible VetChroma Neo assays may support whole-blood testing without centrifugation. Always verify the specimen requirements for your specific test-kit format.

Sample Quality Tip: Properly separated serum should generally appear clear to light yellow. Pink or red discoloration may indicate hemolysis, which can interfere with testing. Re-spinning cannot reverse true hemolysis.

3. Proper Micropipette Technique

Accurate liquid measurement is an important part of progesterone testing.

Most adjustable micropipettes have two plunger positions:

First Stop — Drawing the Sample

Press the plunger to the first stop before placing the tip into the liquid. Slowly release the plunger to aspirate the selected volume.

Second Stop — Dispensing the Sample

To dispense, press the plunger to the first stop, then continue to the second stop to expel the remaining liquid. Withdraw the tip before releasing the plunger.

Helpful Tips:

• Always verify the selected volume before pipetting.

• Use clean, properly fitted disposable tips.

• Aspirate slowly to avoid air bubbles.

• Avoid collecting blood cells when transferring serum.

• Change tips between samples to prevent contamination.

4. Preparing Your Progesterone Test Kit

VetChroma progesterone assays use specially designed reagent components and test cartridges to measure progesterone concentrations.

For compatible VetChroma Neo kits, reagent preparation may involve reconstituting a freeze-dried detector component, combining it with the specimen, and transferring the prepared mixture into the test cartridge.

Basic Preparation Steps:

1. Verify the test-kit expiration date and lot information.

2. Open the required reagent components and cartridge.

3. Add the specified volume of detector diluent to the reagent tube.

4. Mix or dissolve the reagent according to the kit instructions.

5. Add the required amount of serum, plasma, or whole blood, depending on the assay.

6. Mix the prepared sample as directed.

7. Transfer the specified volume into the cartridge's designated sample well.

Use the exact volumes, mixing instructions, and timing provided with your particular test kit. Older VetChroma reagents and current Neo reagents may require different procedures.

5. Running a Progesterone Test on VetChroma 3

Once your sample and cartridge are prepared, you're ready to begin testing.

Step 1 — Prepare the Analyzer

Confirm that the VetChroma 3 is powered on, functioning normally, and ready for the selected progesterone assay.

Step 2 — Load the Cartridge

Insert the prepared test cartridge into an available testing position, following the manufacturer's instructions for cartridge orientation and timing.

Step 3 — Begin Testing

Follow the touchscreen instructions to initiate the test.

The VetChroma 3 features built-in incubation, so compatible assays can be incubated and analyzed directly inside the machine without a separate external incubator.

Step 4 — Review Your Results

After the testing cycle is complete, the analyzer displays the progesterone concentration in ng/mL.

The commonly listed VetChroma canine progesterone assay measures approximately 1–25 ng/mL.

Results can be printed using the machine's integrated printer and recorded for future comparison.

Testing Tip: Record each female's progesterone results by date and time. Comparing serial results makes it easier to monitor hormonal progression throughout the breeding cycle.

6. Common Problems & Troubleshooting

Inconsistent Progesterone Results

Review sample quality, pipetting accuracy, reagent preparation, and test-kit handling. Confirm that quality-control requirements have been met.

Cartridge Not Recognized

Verify cartridge compatibility, lot information, and proper insertion. Never force a cartridge into the analyzer.

Air Bubbles During Pipetting

Aspirate liquid slowly, check that the tip is firmly attached, and inspect the sample before dispensing.

Pink or Red Serum

The sample may be hemolyzed. If it does not meet the assay's specimen requirements, collect a fresh sample.

Analyzer Error Message

Record the displayed error code and consult the operating manual. Contact K9Reproduction for assistance if the issue persists.

Frequently Asked Questions

Does the VetChroma 3 require an external incubator?

No. The VetChroma 3 includes built-in incubation for compatible tests, eliminating the need for a separate cartridge incubator.

How many progesterone tests can the VetChroma 3 run at once?

The analyzer supports up to three compatible tests simultaneously.

Can I use whole blood with VetChroma 3?

Compatible VetChroma Neo progesterone assays support whole-blood testing. Confirm the approved specimen types on your current test-kit instructions.

What is the testing range?

The commonly listed VetChroma canine progesterone assay measures progesterone concentrations from 1–25 ng/mL.

Where can I purchase replacement test kits?

K9Reproduction carries compatible VetChroma progesterone test kits, micropipettes, centrifuges, and laboratory supplies.

VetChroma 3 Test Kits, Supplies & Technical Support

At K9Reproduction, we don't just sell progesterone testing equipment—we use these systems in our working canine reproduction facility.

With over 15 years of hands-on reproduction experience, our team can assist with analyzer setup, compatible test kits, operating questions, and equipment troubleshooting.

Need help with your VetChroma 3?

Call or Text: 951-420-7332

Email: customersupport@k9reproduction.com

Website: www.K9Reproduction.com

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