HealVet 3000 Progesterone Timing Chart for Dogs

If you are using the HealVet 3000 canine progesterone analyzer, understanding what the numbers mean is just as important as running the test correctly. The HealVet 3000 Progesterone Timing Chart for Dogs.

Progesterone testing should be used to follow the rise over several tests, not to make a breeding decision from one isolated number. The HealVet 3000 uses its own testing system and reference ranges, so breeders should follow a chart intended for the HealVet platform rather than comparing results directly with another progesterone analyzer.

HealVet's published material identifies 5–10 ng/mL as the ovulation period and 10–20 ng/mL as the best reproductive period.

HealVet 3000 Progesterone Timing Chart

HealVet 3000 Result	What It Means	What to Do
Below 1 ng/mL	Too early / proestrus	Continue monitoring
1–5 ng/mL	Progesterone is rising	Continue testing
5–10 ng/mL	Ovulation period	Keep testing and follow the rise
10–20 ng/mL	Breeding range / best reproductive period	Breeding commonly takes place in this range
Above 30 ng/mL	Luteal phase	Evaluate based on previous results and breeding timing

HealVet's manufacturer material describes these ranges as <1 ng/mL not in heat or proestrus, 1–5 ng/mL follicular phase, 5–10 ng/mL ovulation period, 10–20 ng/mL best reproductive period, and >30 ng/mL luteal phase.

Below 1 ng/mL: Too Early

A HealVet result below 1 ng/mL is generally still too early in the cycle.

There is usually no reason to begin breeding at this point. Continue monitoring the female and test again as progesterone begins to rise.

1–5 ng/mL: Progesterone Is Rising

Once progesterone moves into the 1–5 ng/mL range, the female is progressing through her cycle.

This is the time to start paying closer attention to the pattern of her results.

For example:

1.8 → 3.2 → 4.7 ng/mL

The upward movement is often more useful than any one number by itself.

Keep testing and chart the rise.

5–10 ng/mL: Ovulation Range

HealVet identifies approximately 5–10 ng/mL as the ovulation period.

This is an important distinction:

Ovulation does not necessarily mean breed immediately.

Canine eggs are released immature and continue maturing after ovulation. CanineP4 also emphasizes that breeding at ovulation itself can be too early and that the timing chart for the specific analyzer should be followed rather than using one universal progesterone number.

So when the HealVet 3000 reaches this range:

Keep testing.

You are looking for the female to continue progressing into the reproductive range.

10–20 ng/mL: Breeding Range

HealVet identifies 10–20 ng/mL as the best reproductive period on its published progesterone chart.

For breeders, this is the main range to pay attention to after ovulation has been established.

That does not mean every female should automatically be bred the second the analyzer displays 10.0.

Look at the complete progression.

For example:

3.4 → 7.1 → 12.8 ng/mL

That tells you considerably more than simply receiving a single result of 12.8.

The female has moved from rising progesterone, through the ovulation range, and into HealVet's reproductive range.

Don't Breed Based on One Number

One of the biggest mistakes with progesterone testing is searching for a single magic number.

There isn't one number that perfectly times every breeding.

Females can:

• rise quickly

• rise gradually

• remain within a range longer than expected

• make a significant jump between tests

That is why we recommend:

Test consistently. Chart the rise. Follow the female.

Fresh, Chilled and Frozen Semen Can Require Different Timing

The type of semen being used also matters.

Natural breeding, fresh semen, chilled semen and frozen semen do not necessarily use identical timing because sperm survival and the desired relationship between insemination and egg maturity can differ.

Your progesterone results should therefore be used together with the female's progression and the breeding method you are using.

Stay With the Same Progesterone Analyzer

Do not assume a result from the HealVet 3000 should match a Wondfo, VetChroma, Mini VIDAS or another progesterone analyzer number-for-number.

CanineP4 specifically notes that progesterone systems can produce different numerical results on the same canine serum sample and recommends using the timing chart developed for the analyzer being used.

If you start the cycle on a HealVet 3000, it is best to continue charting that cycle on the same HealVet system whenever possible.

HealVet 3000 Progesterone Testing

The HealVet 3000 is a quantitative veterinary fluorescence immunoassay analyzer. HealVet lists the analyzer as a compact system with an internal printer and touchscreen interface.

For the canine progesterone assay, published HealVet material lists:

Sample: Serum or EDTA plasmaSample volume: 75 µLTest time: Approximately 15 minutesMeasurement range: 1–50 ng/mL

This allows breeders to perform quantitative progesterone testing in-house rather than relying exclusively on outside testing.

The Simple HealVet 3000 Takeaway

Remember these three areas:

Below 5 ng/mL: Keep testing.

5–10 ng/mL: Ovulation range — keep following the rise.

10–20 ng/mL: HealVet's published reproductive/breeding range.

Most importantly:

Chart the female, not just the number.

Progesterone testing is much easier to understand once you stop looking for one perfect result and start following the progression of the entire cycle.

K9 Reproduction carries the HealVet 3000 canine progesterone analyzer, progesterone test kits and testing supplies for breeders interested in bringing progesterone testing in-house.

Visit K9Reproduction.com to view current HealVet equipment, test kits and available packages.