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Mastering the Progesterone Test on Dogs: A Vital Tool for Breeders

Reviewed by: Dr. Alicia Martinez, DVM

Accurate progesterone testing is a cornerstone of successful canine breeding programs. If you're searching for information on "progesterone test on dogs," this comprehensive guide will provide you with the essential knowledge and practical insights needed to optimize breeding outcomes.

The Importance of Progesterone Testing in Dogs

Progesterone is a hormone crucial to the reproductive cycle in dogs. Monitoring its levels allows breeders and veterinarians to determine the precise timing for breeding, which greatly enhances the chances of successful conception.

How Progesterone Testing Works

Understanding Progesterone Levels

Progesterone levels in female dogs rise sharply before ovulation, providing a clear indication of the optimal breeding time. By regularly testing these levels, breeders can pinpoint when a dog is most fertile.

The Testing Process

  1. Blood Sample Collection

  • A small blood sample is drawn, typically from a vein in the dog's foreleg.

  • This sample is then sent to a lab or analyzed on-site using specialized equipment.

  1. Laboratory vs. In-House Testing

  • Laboratory Testing: Offers high accuracy and detailed results but takes longer.

  • In-House Testing: Provides quick results, allowing immediate decision-making, but requires proper equipment and trained personnel.

Real-World Application: Case Study of a Successful Breeding

Consider Sarah, a breeder who owns a German Shepherd named Luna. Sarah aims to maximize her breeding program's success using progesterone testing.

  1. Monitoring Heat Cycle:

  • Sarah notices Luna’s heat cycle starting with signs like vulva swelling and discharge. She schedules the first progesterone test.

  1. Initial Testing:

  • The initial test shows progesterone levels at 0.8 ng/mL, indicating it’s too early for breeding.

  1. Subsequent Tests:

  • Over the next few days, Sarah continues testing every two days. On the fourth test, Luna’s progesterone levels rise to 2.5 ng/mL.

  1. Optimal Breeding Time:

  • The vet advises that the optimal breeding window is near. Luna’s progesterone levels reach 5 ng/mL on the sixth test, confirming it’s the perfect time for mating.

  1. Successful Outcome:

  • Luna successfully conceives, resulting in a healthy litter, thanks to precise timing facilitated by progesterone testing.

Advanced Techniques and Best Practices

Using Progesterone Analyzers

Modern veterinary practices utilize progesterone analyzers to provide rapid and accurate results. These machines, like the Vet Chroma, allow breeders to make timely decisions without waiting for external lab results.

Best Practices for Testing

  1. Early and Regular Testing:

  • Begin testing a few days after the onset of estrus and continue every two days.

  • Regular monitoring helps avoid missing the critical breeding window.

  1. Accurate Record-Keeping:

  • Maintain detailed records of progesterone levels and corresponding dates.

  • Use these records to predict future heat cycles and optimize breeding schedules.

  1. Consultation with Veterinarians:

  • Work closely with a veterinarian to interpret test results accurately and make informed breeding decisions.

Frequently Asked Questions (FAQs)

Q1: When should I start progesterone testing on my dog? A1: Start testing a few days after the onset of estrus (heat) and continue every two days until the optimal breeding window is identified.

Q2: How is progesterone testing conducted? A2: A blood sample is collected from the dog and analyzed either in-house using a progesterone analyzer or sent to a laboratory.

Q3: What do different progesterone levels indicate? A3: Levels below 1 ng/mL indicate it’s too early to breed. Levels between 2-5 ng/mL suggest ovulation is imminent, and levels above 5 ng/mL indicate that ovulation has occurred.

Q4: Is progesterone testing expensive? A4: The cost varies but typically ranges from $50 to $100 per test, depending on whether it’s done in-house or through a lab.

Q5: Can progesterone testing be done at home? A5: While at-home kits exist, it’s recommended to have a veterinarian perform the tests to ensure accuracy and proper interpretation.

Booking an Appointment for Progesterone Testing

At our Menifee, CA office, we offer comprehensive progesterone testing services to ensure your breeding program's success. Here’s how you can book an appointment:

  1. Contact Us: Call or email our office to schedule an initial consultation.

  2. Initial Consultation: Discuss your breeding goals and evaluate the best timing for progesterone testing.

  3. Schedule Tests: Based on the initial consultation, we’ll schedule the progesterone tests at the optimal times.


Progesterone testing is an invaluable tool for breeders aiming to optimize their breeding programs. By understanding the reproductive cycle and accurately timing breeding, you can significantly increase the chances of successful conception. Whether using in-house analyzers or laboratory services, the key is to start testing early and continue until the optimal breeding window is identified.

For those searching for "progesterone test on dogs," our Menifee, CA office offers comprehensive services to meet your needs. Book an appointment with us today to ensure the success of your breeding program. Contact us at [Your Contact Information] to schedule your consultation and progesterone tests.

For more information about our services and training programs, visit

Wondfo Progesterone Test on Dogs
Wondfo Progesterone Test on Dogs


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