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Guidelines for Blood Collection in Dogs with Focus on Canine Reproduction and Progesterone Testing

At, our expertise in supporting canine health and reproductive care emphasizes precise and considerate blood collection in dogs. This practice is crucial for breeding purposes, where progesterone testing plays a key role. Below, we delve into the best practices for blood collection in dogs, highlighting the use of advanced diagnostic tools like the Wondfo Pro-DX and the Vet Chroma Canine Progesterone Machine.

Understanding Blood Volume for Effective Blood Collection in Dogs

The total blood volume in dogs generally ranges from 55-70 ml per kg of body weight. This estimation is crucial for safe blood collection in dogs and varies depending on factors such as age and body condition, with older or obese dogs having slightly lower blood volumes.

Blood Collection in Dogs
Blood Collection in Dogs

Best Practices for Blood Collection in Dogs

To ensure the health and safety of dogs during blood collection, adhere to these specific guidelines:

  • Maximum Single Draw: No more than 1% of the dog's body weight should be drawn for blood collection in dogs every 14 days, which avoids the need for fluid replacement.

  • Daily Collection: Daily blood collection in dogs should not exceed 0.07% of the dog's body weight to prevent dehydration and health complications.

  • Multiple Draws Over Two Weeks: Guidelines for multiple sessions of blood collection in dogs within a 14-day period include:

  • Up to 1% of body weight: No extra measures needed.

  • Up to 2% of body weight: Fluid replacement is necessary to maintain proper hydration and health.

Progesterone Testing and Its Importance in Blood Collection in Dogs for Canine Reproduction

Progesterone testing is crucial in managing breeding programs and understanding the reproductive cycles in dogs. The precision of blood collection in dogs for progesterone testing, facilitated by devices like the Wondfo Pro-DX or the Vet Chroma Canine Progesterone Machine, allows breeders to accurately determine the optimal breeding times. These devices are designed to require minimal blood, making the process less invasive and more efficient.

Monitoring and Health Safety in Blood Collection in Dogs

Monitoring a dog's condition after blood collection is crucial. Symptoms of hypovolemic shock, such as a rapid pulse, pale mucous membranes, and hyperventilation, necessitate immediate veterinary intervention. Regular assessments of hematocrit and hemoglobin levels are essential after blood collection in dogs to ensure their health and readiness for further procedures.

Special Considerations for Blood Collection in Dogs

  • Compromised Dogs: Dogs that are stressed, sick, or otherwise compromised may not tolerate typical volumes for blood collection. Such animals require closer monitoring and potentially more conservative approaches to blood collection.

  • Recovery Considerations: Following blood collection in dogs, recovery indicators such as hematocrit and hemoglobin levels might take up to 24 hours to normalize.

Conclusion to Blood Collection in Dogs

At, we are dedicated to ensuring that blood collection in dogs is performed safely, humanely, and effectively, contributing to optimal health and successful breeding outcomes. Our commitment to advanced technology for progesterone testing and reproduction management in dogs underscores our dedication to the welfare of these animals and the success of breeders. Blood Collection in Dogs


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