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The Comprehensive Guide to Using a Progesterone Machine for Dogs

Monitoring progesterone levels is a critical component of successful canine breeding. A progesterone machine for dogs, such as the Wondfo and Vet Chroma devices, provides breeders and veterinarians with precise and timely hormone level measurements. This comprehensive guide will offer detailed insights, practical techniques, and real-world experiences related to using a progesterone machine for dogs. We will also address common questions and concerns through a detailed FAQ section.

Why Use a Progesterone Machine?

Progesterone testing is essential for determining the optimal breeding time for female dogs. Accurate progesterone measurements can significantly increase the chances of successful conception by identifying the precise moment of ovulation.

Understanding Progesterone Machines for Dogs

Key Features

  1. Accuracy and Precision:

  • Progesterone machines, like the Wondfo and Vet Chroma, are designed to provide accurate and precise hormone level readings.

  • These devices can detect even slight changes in progesterone levels, ensuring the best timing for breeding.

  1. Ease of Use:

  • Modern progesterone machines are user-friendly, with straightforward interfaces and easy-to-follow instructions.

  • Both the Wondfo and Vet Chroma machines are designed for efficient operation in a clinical or breeding setting.

  1. Quick Results:

  • These machines deliver rapid results, often within minutes, allowing breeders and veterinarians to make timely decisions.

  • Quick turnaround times are crucial for scheduling insemination procedures accurately.

Using a Progesterone Machine: Step-by-Step

  1. Blood Sample Collection:

  • Collect a small blood sample from the dog, usually from a vein in the foreleg.

  • Ensure the sample is handled properly to avoid contamination or degradation.

  1. Preparing the Machine:

  • Turn on the progesterone machine and follow the manufacturer's instructions for calibration and setup.

  • Load the blood sample into the machine using the provided cartridges or test strips.

  1. Running the Test:

  • Initiate the test according to the machine’s instructions.

  • The machine will analyze the sample and provide a progesterone level reading.

  1. Interpreting Results:

  • Compare the progesterone levels to the standard breeding timeline:

  • Levels below 1 ng/mL: Not yet ready for breeding.

  • Levels between 2-5 ng/mL: Ovulation is imminent; ideal time for breeding.

  • Levels above 5 ng/mL: Ovulation has occurred; immediate breeding is recommended.

Real-World Scenario: Successful Breeding with a Progesterone Machine

Consider Lisa, a breeder who owns a prized French Bulldog named Bella. Lisa wants to ensure Bella’s breeding is timed perfectly to maximize the chances of a successful litter. She uses a Vet Chroma progesterone machine to monitor Bella’s hormone levels.

  1. Initial Testing:

  • Lisa notices signs of heat and starts testing Bella’s progesterone levels. The initial reading is 0.8 ng/mL.

  1. Regular Monitoring:

  • Lisa tests Bella every two days. On the third test, Bella’s progesterone levels rise to 3.2 ng/mL.

  1. Optimal Breeding Time:

  • With progesterone levels indicating imminent ovulation, Lisa schedules the breeding procedure.

  1. Successful Outcome:

  • Bella successfully conceives and delivers a healthy litter, thanks to precise timing facilitated by the Vet Chroma machine.

Best Practices for Using Progesterone Machines

  1. Ensure Proper Training:

  • Familiarize yourself with the machine’s operation by reviewing the user manual and receiving training if necessary.

  1. Maintain Sterility:

  • Always use sterile equipment and handle blood samples carefully to avoid contamination.

  1. Regular Calibration:

  • Ensure the machine is regularly calibrated and maintained according to the manufacturer’s guidelines.

  1. Consult a Veterinarian:

  • Work closely with a veterinarian to interpret results and make informed breeding decisions.

Frequently Asked Questions (FAQs)

Q1: How accurate are progesterone machines for dogs? A1: Progesterone machines like Wondfo and Vet Chroma are highly accurate, providing precise hormone level readings crucial for determining optimal breeding times.

Q2: Can I use a progesterone machine at home? A2: While some breeders may use these machines at home, it is recommended to have a veterinarian or trained professional handle the testing to ensure accuracy and proper interpretation.

Q3: What is the cost of a progesterone machine for dogs? A3: The cost varies depending on the brand and model, typically ranging from $1,500 to $5,000. Additional costs include test cartridges and maintenance.

Q4: How often should I test my dog's progesterone levels? A4: Begin testing a few days after the onset of heat and continue every two days until the optimal breeding window is identified.

Q5: Are there any risks associated with progesterone testing? A5: The procedure is minimally invasive, with the primary risk being minor discomfort during blood sample collection.


Progesterone machines are invaluable tools for breeders aiming to optimize breeding outcomes. By providing accurate and timely hormone level measurements, these machines help pinpoint the best time for breeding, significantly increasing the chances of successful conception. Whether you are a professional breeder or a veterinarian, incorporating a progesterone machine into your practice can greatly enhance your breeding program.

For those seeking more information or training on using progesterone machines like Wondfo and Vet Chroma, visit to explore our comprehensive courses and resources.

Progesterone Machine for Dogs
Progesterone Machine for Dogs


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