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What is Progesterone testing for Canines and why do we test?

Progesterone testing is a crucial tool for dog breeders. It helps to determine the ideal time for breeding and can increase the chances of a successful pregnancy. In this article, we will discuss the facts and benefits of progesterone testing for dogs.

What is Progesterone Testing?

Progesterone is a hormone that plays a vital role in a dog's reproductive cycle. It helps to prepare the uterus for pregnancy and maintains the pregnancy after conception. Progesterone testing measures the level of progesterone in a dog's blood. The results of the test help to predict the best time for breeding and can indicate whether a dog is ready top give birth or not.

Benefits of Progesterone Testing

There are several benefits to progesterone testing for dogs. These include:

  1. Predicting ovulation: Progesterone levels rise as a dog approaches ovulation. By monitoring progesterone levels, breeders can predict the best time for breeding to increase the chances of pregnancy.

  2. Identifying the best time for breeding: Breeding at the right time can increase the chances of a successful pregnancy. Progesterone testing can help breeders determine the optimal time for breeding.

  3. Increasing the chances of pregnancy: By breeding at the right time, the chances of a successful pregnancy are increased. This can reduce the risk of miscarriage and stillbirth.

  4. Monitoring pregnancy: Progesterone testing can also be used to monitor pregnancy. If progesterone levels drop suddenly, it may indicate a problem with the pregnancy, such as a miscarriage or a dead puppy.

  5. Timing of Birth/Cesarean. This can be an especially important tool for dogs that are not able to give birth naturally. This can also help determine whether it is okay to leave the home or not.

Timing of Progesterone Testing

The timing of progesterone testing is critical. In general, breeders should start testing a female dog's progesterone levels around day seven of her heat cycle. The frequency of testing will depend on the breed and individual dog, but it is usually recommended to test every two to three days until ovulation occurs. Once ovulation has been detected, breeders should continue to test until progesterone levels have peaked.

Interpreting Progesterone Test Results

Interpreting progesterone test results can be complicated, and it is essential to work with a reproduction specialist or experienced breeder. The ideal progesterone level for breeding varies depending on the breed, but in general, levels should be above 13 ng/mL. When levels have peaked after ovulation, breeding should occur within 48 hours.

Risks of Breeding Without Progesterone Testing

Breeding without monitoring a dog's progesterone levels can result in missed breeding opportunities, low pregnancy rates, and an increased risk of pregnancy complications. Without knowing the optimal time for breeding, the chances of a successful pregnancy are greatly reduced. In some cases, breeding at the wrong time can even be dangerous for the female dog and her puppies.

Finding a Reliable Progesterone Testing Service

When looking for a progesterone testing service, it is essential to find a reliable and experienced provider. Ask for recommendations from other breeders, check online reviews, and research the testing methods used. Make sure the provider is using a reliable and accurate testing method.


Progesterone testing is an essential tool for dog breeders. By monitoring a female dog's progesterone levels, breeders can predict the best time for breeding, increase the chances of a successful pregnancy, and reduce the risk of complications. It is essential to work with a veterinarian or reproduction specialist when interpreting progesterone test results and to find a reliable testing service. With the right care and attention, breeders can help to ensure the health and wellbeing of their dogs and their puppies.


Brytany Hayes
Brytany Hayes
Jul 20, 2023

You’re welcome and thank you so much for the compliment 😊


Thank you for sharing your cutting edge info on your latest equipment and testing 👍👍

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