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Progesterone and Blood Analyzers 

Canine progesterone testing is a pivotal aspect of modern canine reproduction management, offering breeders and veterinary professionals a scientific basis to make informed decisions regarding breeding timing, surgical insemination, and the timing of Cesarean sections (C-sections). At, we specialize in providing the latest and most accurate canine progesterone testing equipment and supplies essential for optimizing the breeding process and ensuring the health and well-being of both the breeding females and their offspring. 

Understanding Canine Breeding Cycles

The canine estrous cycle consists of several stages, with the timing of ovulation being crucial for successful mating. Progesterone, a hormone produced by the ovaries after ovulation, plays a key role in preparing the dog's uterus for pregnancy and maintaining pregnancy. Monitoring progesterone levels with canine progesterone testing equipment allows breeders and veterinarians to pinpoint the optimal time for mating or insemination. 

The Role of Progesterone Testing

Optimal Breeding Timing: Progesterone testing is instrumental in determining the precise moment ovulation occurs, which is when the female is most fertile. The levels of progesterone rise markedly close to ovulation, and by measuring these levels, one can accurately predict the best time for breeding to occur. This significantly enhances the chances of conception and a successful pregnancy.

Timing of C-Sections: In canine reproduction, the timing of C-sections is critical to ensure the health and safety of the mother and puppies. Progesterone levels drop significantly before the onset of labor. By closely monitoring these levels, veterinarians can decide the most appropriate time to perform a C-section, minimizing risks and improving outcomes for the dam and her litter.

Surgical Insemination Timing: For procedures such as Transcervical Insemination (TCI) or surgical insemination, knowing the exact timing of ovulation is paramount. Progesterone testing facilitates the identification of this fertile window, thereby maximizing the success rate of these advanced reproductive techniques.

Our Canine Progesterone Testing Equipment

At, we are committed to accuracy and reliability in progesterone testing. Our analyzers are clinically accurate, providing breeders and veterinary professionals with the confidence to make timely and informed decisions regarding canine breeding and healthcare. Our product range caters to all needs, from small-scale breeders to full-scale veterinary practices, ensuring that every client finds the perfect tool for their specific requirements.

The k9reproduction Satisfaction Guarantee

Our dedication to quality and customer satisfaction is reflected in the k9reproduction satisfaction guarantee. We understand the importance of reliable progesterone testing in the breeding process and are committed to providing products that breeders and veterinarians can trust.

Save Time and Money

By integrating our progesterone testing solutions into your breeding program or veterinary practice, you can significantly reduce the cost and time associated with external veterinary visits for progesterone testing. Our in-house testing solutions offer a convenient, reliable, and cost-effective alternative, allowing for more frequent and timely testing without the added expense.

Get Started Today

Visit to explore our extensive range of canine progesterone analyzers and testing supplies. Our expert team is available to assist you in selecting the ideal solution for your breeding program or practice, ensuring you have the tools necessary to achieve successful outcomes. Choose for your canine progesterone testing needs and join the many satisfied customers who have experienced the difference our products and expertise can make in the field of canine reproduction.


The outlined progesterone scale provides a structured framework for interpreting the stages of the estrous cycle in canines, especially in relation to breeding management. This framework is particularly tailored to users of the Vet Chroma Canine Progesterone Analyzer, although the principles of progesterone measurement and its implications for breeding are broadly applicable across different analyzer models. Each stage identified by specific progesterone levels offers insights into the reproductive status of the female dog, guiding optimal breeding times.

LH Surge (2.0 ng/mL - 4.9 ng/mL)

The initial rise in progesterone levels from baseline (typically below 2.0 ng/mL) to this range indicates the onset of the LH surge. The luteinizing hormone (LH) surge is a critical reproductive marker, signaling the beginning of ovulation processes. Ovulation typically occurs 48-72 hours post-LH surge. This stage is crucial for identifying the impending fertile window but is considered early for breeding purposes.

Post LH Surge, Pre-Ovulation (5.0 ng/mL - 6.9 ng/mL)

Following the LH surge, progesterone levels continue to rise, indicating the approach of ovulation. This phase signifies that the follicles are maturing but have not yet released the oocytes (eggs). Breeding during this phase is premature, as the oocytes require time post-ovulation to reach maturity for fertilization.

Near Ovulation (7.0 ng/mL - 12.0 ng/mL)

This range suggests that ovulation is imminent or occurring. Oocytes are released from the follicles and will mature over the next 48 hours, making this a critical window for planning insemination. The higher end of this range (close to 12.0 ng/mL) is particularly optimal for timing the first breeding, as it aligns closely with the oocytes' maturation period.

Determining Ovulation Occurred (13.0 ng/mL - 15.0 ng/mL)

Levels within this range indicate that ovulation has likely occurred. The oocytes are in the process of maturing, which takes approximately 48 hours post-release. This period is optimal for the first breeding attempt, ensuring sperm are present as the oocytes reach fertilizable maturity.

First Breeding (15.0 ng/mL - 20.0 ng/mL)

Progesterone levels within this range are ideal for the first breeding. The oocytes have matured and are at their most fertilizable state. Scheduling the first breeding session here maximizes the likelihood of successful fertilization.

Second Breeding (21.0 ng/mL - 25.0 ng/mL)

A second breeding, suggested in this progesterone range, takes advantage of the continued presence of viable oocytes and the female's receptivity. This timing ensures that any oocytes which were not fertilized during the first breeding have another opportunity for fertilization, optimizing the chances for a successful pregnancy.

Third Breeding (≥24.0 ng/mL)

Continuing to breed at progesterone levels of 24 ng/mL and above may be beneficial in certain breeding strategies, particularly where maximizing the genetic diversity of a litter is desired. However, it's important to note that the window for successful fertilization narrows as progesterone levels increase significantly beyond this point.


Understanding and interpreting these progesterone levels using a reliable analyzer like the Vet Chroma Canine Progesterone Analyzer allows veterinary professionals and breeders to make informed decisions about the timing of breeding. Accurate timing is crucial for maximizing the chances of conception and ensuring the health and well-being of the breeding female and her offspring. It's important to follow the manufacturer's guidelines for the specific analyzer being used, as scales and recommendations may vary slightly between models. This approach, grounded in veterinary science, enhances the success rates of canine breeding programs by aligning breeding actions with the physiological stages of the female dog's reproductive cycle.

Mastering Canine Progesterone Testing with VetChroma™: A Step-by-Step Guide

In the realm of canine reproduction, understanding and mastering the use of the VetChroma™ Canine Progesterone Analyzer is crucial for breeders and veterinary professionals. This high-precision tool not only streamlines the breeding process but also ensures the health and well-being of dogs. Here, we provide a detailed walkthrough of conducting a progesterone test with VetChroma™ and introduce our comprehensive training section designed to optimize your proficiency with this innovative device.

Conducting a Test with VetChroma™: Simplified Precision

The VetChroma™ Canine Progesterone Analyzer is engineered for simplicity and accuracy, making it an indispensable tool in your breeding toolkit. Here’s how to conduct a test effectively:

  1. Sample Collection: Begin with collecting a blood sample from the dog using the blood collection essentials provided. This step is crucial for ensuring the accuracy of the test results.

  2. Sample Preparation: Utilize the centrifuge (recommended for optimal preparation) to separate the serum from the blood. This serum is what will be analyzed for progesterone levels.

  3. Testing Process: With the serum prepared, use the pipette to place the sample into the VetChroma™ analyzer. The device's user-centric design and intuitive interface make this process straightforward, ensuring that you can conduct the test with ease.

  4. Reading Results: Within minutes, VetChroma™ delivers precise progesterone measurements. These results are critical for identifying the optimal breeding window, managing C-sections, and ensuring the overall reproductive health of your canine.

Elevate Your Skills with VetChroma™ Training

To ensure that you can leverage the full potential of VetChroma™, we’ve developed an extensive training section. This resource is designed to enhance your understanding and skills in using the VetChroma™ analyzer effectively. Whether you're a seasoned breeder or a veterinary professional, our training materials provide valuable insights and practical tips for mastering progesterone testing.

Why Training Matters

  • Maximize Efficiency: Learn how to conduct tests more swiftly and accurately, maximizing the efficiency of your breeding program.

  • Ensure Accuracy: Proper training reduces the risk of errors, ensuring the reliability of your test results.

  • Informed Decision Making: Understanding the nuances of progesterone levels and their implications on breeding decisions is crucial for success.


The VetChroma™ Canine Progesterone Analyzer is a game-changer in canine reproduction, offering unmatched precision and ease of use. By following our step-by-step guide and taking advantage of the training resources available, you can master the art of progesterone testing. This knowledge not only empowers you to make informed breeding decisions but also enhances the health and well-being of the dogs in your care.

Unlock the full potential of VetChroma™ and elevate your breeding program to new heights. For more information on our training resources or to get started with VetChroma™, visit our website or contact us directly. Embrace the future of canine reproductive diagnostics with VetChroma™ – where technology, training, and tradition converge for excellence in breeding.

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